Tth DNA Polymerase

EC Number

EC 2.7.7.7

Origin: Escherichia coli, Recombinant

Usage
The enzyme has a reverse transcriptase activity in addition to a 5’→3’ polymerase activity and a double strand specific 5’→3’ exonuclease activity in the presence of Mn2+ ions.

Key Specifications

Concentration/Activity*: 5U/µL

Purity: ≥90%

Ribonuclease Activity: None detected

Endonuclease Activity: None detected

Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.

Key Characteristics

Exhibits reverse transcriptase activity in the presence of Mn2+ ions

Effective for the amplification of GC-rich targets and crude samples

Effective for reverse transcription of RNA with complicated secondary structure due to the reaction occurring at high temperature (i.e. 60°C)

Product Available in select countries in the following territories:

Asia (only in select countries), Europe, USA

Ordering Information

Name	Size	Part	Package Insert
10x Buffer for Tth	1 mL 10x Reaction Buffer for Tth	TTH-3R	N/A
Tth DNA Polymerase Kit	0.05 mL (250U) Tth DNA Polymerase, 1mL 10x Reaction Buffer for Tth, 1mL Dilution Buffer, 1mL 2mM dNTPs	TTH-301	N/A
Tth DNA Polymerase 10KU	2mL (10KU) Tth DNA Polymerase	TTH-329L	N/A

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