TTx DNA Polymerase

TTx DNA Polymerase exhibits reverse transcription activity in the presence of Mn2+ ions and allows for 1-Step 1-Enzyme RT-PCR, including reverse transcription and PCR steps.

TTx DNA Polymerase

Origin: Escherichia coli, Recombinant

Usage
Effective for amplification from crude samples containing PCR inhibitors with high efficiency for both DNA and RNA. Suitable for amplification from low copies of template DNA/RNA and is efficient even in fast cycle condition.
In addition, TTx DNA Polymerase has a 5 ‘- 3’ exonuclease activity, so it can be used for real-time PCR using probe assays such as TaqMan® assay.

Key Specifications

Enzyme Specification

  • Ribonuclease Activity: None detected
  • Endonuclease Activity: None detected
  • Nicking Activity: None detected

Hot Start TTx [DNA/RNA] Kit Specification

  • Tested for functional performance by amplifying from dilutions of enterovirus RNA in combination with 5x Buffer for rTth/ TTx (DNA/ RNA)
  • The slope from analysis is as follows: -3.74 ≤ slope ≤ -3.01
  • The correlation coefficients from both analyses are as follows: r ≤ -0.980

Key Characteristics

  • Enables highly efficient 1-enzyme 1-step RT-PCR
  • Effective amplification from crude samples
  • Fast- High speed qPCR cycling detection
  • Suitable for Multiplex PCR
Product Available in select countries in the following territories:

Asia, Europe, USA

Ordering Information

Name Size Part Package Insert
TTx DNA Polymerase TTx DNA Polymerase 1KU TTX-119 N/A
TTx DNA Polymerase TTx DNA Polymerase 10KU TTX-129 N/A
TTx DNA Polymerase TTx DNA Polymerase 100KU TTX-159 N/A
TTx DNA Polymerase (Glycerol free) TTx DNA Polymerase (Glycerol free) 1KU TTX-219 N/A
TTx DNA Polymerase (Glycerol free) TTx DNA Polymerase (Glycerol free) 10KU TTX-229 N/A
TTx DNA Polymerase (Glycerol free) TTx DNA Polymerase (Glycerol free) 100KU TTX-259 N/A
Hot Start TTx [DNA] kit Hot Start TTx DNA Polymerase (4U/uL) 62.5uL; 2x Buffer for rTth/TTx (DNA) 1.25mL x2 HSTTX-101 N/A
Hot Start TTx [DNA] kit Hot Start TTx DNA Polymerase (4U/uL) 62.5uL; 5x Buffer for rTth/TTx (DNA/RNA) 1mL; 50mM Mn (OAc)2 250uL; 2mM dNTPs 1 mL HSTTX-111 N/A

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