Tth DNA Polymerase

Tth DNA Polymerase

EC Number

EC 2.7.7.7

Origin: Escherichia coli, Recombinant

Usage
The enzyme has a reverse transcriptase activity in addition to a 5’→3’ polymerase activity and a double strand specific 5’→3’ exonuclease activity in the presence of Mn2+ ions.

Key Specifications

• Concentration/Activity*: 5U/µL
• Purity: ≥90%
• Ribonuclease Activity: None detected
• Endonuclease Activity: None detected
• Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.

Key Characteristics

• Exhibits reverse transcriptase activity in the presence of Mn2+ ions
• Effective for the amplification of GC-rich targets and crude samples
• Effective for reverse transcription of RNA with complicated secondary structure due to the reaction occurring at high temperature (i.e. 60°C)

Asia (only in select countries), Europe, USA