Usage
The enzyme has a reverse transcriptase activity in addition to a 5’→3’ polymerase activity and a double strand specific 5’→3’ exonuclease activity in the presence of Mn2+ ions.
Key Specifications
Concentration/Activity*: 5U/µL
Purity: ≥90%
Ribonuclease Activity: None detected
Endonuclease Activity: None detected
Nicking Activity: None detected
*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.
Key Characteristics
Exhibits reverse transcriptase activity in the presence of Mn2+ ions
Effective for the amplification of GC-rich targets and crude samples
Effective for reverse transcription of RNA with complicated secondary structure due to the reaction occurring at high temperature (i.e. 60°C)