Tth DNA Polymerase

Tth DNA polymerase is a thermostable DNA polymerase derived from the thermophilic bacteria Thermus thermophilus (Tth) HB8.

Tth DNA Polymerase

EC Number

EC 2.7.7.7

Origin: Escherichia coli, Recombinant

Usage
The enzyme has a reverse transcriptase activity in addition to a 5’→3’ polymerase activity and a double strand specific 5’→3’ exonuclease activity in the presence of Mn2+ ions.

Key Specifications

  • Concentration/Activity*: 5U/µL
  • Purity: ≥90%
  • Ribonuclease Activity: None detected
  • Endonuclease Activity: None detected
  • Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.

Key Characteristics

  • Exhibits reverse transcriptase activity in the presence of Mn2+ ions
  • Effective for the amplification of GC-rich targets and crude samples
  • Effective for reverse transcription of RNA with complicated secondary structure due to the reaction occurring at high temperature (i.e. 60°C)

 

Product Available in select countries in the following territories:

Asia (only in select countries), Europe, USA

Ordering Information

Name Size Part Package Insert
10x Buffer for Tth 1 mL 10x Reaction Buffer for Tth TTH-3R N/A
Tth DNA Polymerase Kit 0.05 mL (250U) Tth DNA Polymerase, 1mL 10x Reaction Buffer for Tth, 1mL Dilution Buffer, 1mL 2mM dNTPs TTH-301 N/A
Tth DNA Polymerase 10KU 2mL (10KU) Tth DNA Polymerase TTH-329L N/A

Pricing and ordering details are determined by several factors.  Please provide your contact information and we will be in touch shortly with more detailed product information.

Get in Touch

When you’re ready to order, we’re here to help.

Fill out the form below and someone from our team will get back to you shortly.