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Taq DNA Polymerase

Taq DNA polymerase is the most widely used thermostable DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq ) YT-1.

Ordering Details

Taq DNA Polymerase

EC Number

EC 2.7.7.7

Origin: Escherichia coli, Recombinant

Usage
The enzyme possesses a 5’→3’ polymerase activity and a double-strand specific 5’→3’ exonuclease activity.

Key Specifications

Enzyme Specification

  • Concentration/Activity*: 5U/µL
  • Purity: ≥90%
  • Endonuclease Activity: None detected
  • Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.

Hot Start Antibody Specification

  • Source: Mouse Hybridoma
  • Concentration: 1mg/mL
  • Inhibition Activity**: ≥95%

Mouse Genomic DNA Contamination: None detected
**Percentage (%) of DNA polymerase activity suppressed when 1 µG is added to 5 units of Taq DNA Polymerase at 40°C

Key Characteristics

  • Tolerates various kinds of PCR protocols
  • Applicable for hot start technology by adding anti-Taq antibody
  • PCR products can be cloned by using a TA cloning method
  • Incorporates dUTP, dITP, and fluorescently-labelled nucleotides
Locations that Product is Available

Asia (only in select countries), Europe, USA

Documentation

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Ordering Information

Name Size Part Package Insert
Taq DNA Polymerase Kit A 50μL (250U) Taq DNA Polymerase, 1mL 10x Buffer (+Mg) for Taq, 1mL 2mM dNTPs TAP-211 N/A
Taq DNA Polymerase 10KU 2mL (10KU) Taq DNA Polymerase TAP-229E N/A
Taq DNA Polymerase 100KU 20mL (100KU) Taq DNA Polymerase TAP-259E N/A
10x Buffer (+Mg) for Taq 1 mL 10x Buffer (+Mg) for Taq TAP-2M N/A
HS Antibody (Taq) Kit 0.1mL (0.1mg) anti-Taq high, 1mL 10x PCR Buffer anti-Taq high TCP-101 N/A
HS Antibody (Taq) 30mg 30mL anti-Taq high TCP-139 N/A
HS Antibody (Taq) 100mg 100mL anti-Taq high TCP-189CH1 N/A

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