Taq DNA Polymerase

EC Number

EC 2.7.7.7

Origin: Escherichia coli, Recombinant

Usage
The enzyme possesses a 5’→3’ polymerase activity and a double-strand specific 5’→3’ exonuclease activity.

Key Specifications

Enzyme Specification

Concentration/Activity*: 5U/µL

Purity: ≥90%

Endonuclease Activity: None detected

Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 minutes at 75°C.

Hot Start Antibody Specification

Source: Mouse Hybridoma

Concentration: 1mg/mL

Inhibition Activity**: ≥95%

Mouse Genomic DNA Contamination: None detected
**Percentage (%) of DNA polymerase activity suppressed when 1 µG is added to 5 units of Taq DNA Polymerase at 40°C

Key Characteristics

Tolerates various kinds of PCR protocols

Applicable for hot start technology by adding anti-Taq antibody

PCR products can be cloned by using a TA cloning method

Incorporates dUTP, dITP, and fluorescently-labelled nucleotides

Product Available in select countries in the following territories:

Asia (only in select countries), Europe, USA

Ordering Information

Name	Size	Part	Package Insert
Taq DNA Polymerase Kit A	50μL (250U) Taq DNA Polymerase, 1mL 10x Buffer (+Mg) for Taq, 1mL 2mM dNTPs	TAP-211	N/A
Taq DNA Polymerase 10KU	2mL (10KU) Taq DNA Polymerase	TAP-229E	N/A
Taq DNA Polymerase 100KU	20mL (100KU) Taq DNA Polymerase	TAP-259E	N/A
10x Buffer (+Mg) for Taq	1 mL 10x Buffer (+Mg) for Taq	TAP-2M	N/A
HS Antibody (Taq) Kit	0.1mL (0.1mg) anti-Taq high, 1mL 10x PCR Buffer anti-Taq high	TCP-101	N/A
HS Antibody (Taq) 30mg	30mL anti-Taq high	TCP-139	N/A
HS Antibody (Taq) 100mg	100mL anti-Taq high	TCP-189CH1	N/A

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Taq DNA Polymerase

Taq DNA Polymerase

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