RNase Inhibitor

This product is a recombinant type human ribonuclease inhibitor purified using a combination of ion exchange and affinity chromatography.

RNase Inhibitor

EC Number

EC 2.7.7.49

Origin: Escherichia coli, Recombinant

Usage
This inhibitor exhibits broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, and RNase H. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. This inhibitor can be applied to a reverse transcriptase reaction.

Key Specifications

  • Concentration/Activity**: 40 U/µl
  • Purity: ≥90%
  • RNase Activity: None detected
  • Endonuclease Activity: None detected
  • Exonuclease activity: None detected

** One unit is defined as the amount of RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50 %.

Key Characteristics

  • Stabilizes RNA in a cDNA synthesis or a RNA synthesis reaction
  • Does not inhibit SP6, T7 or T3 RNA Polymerase, AMV or M-MLV Reverse Transcriptase, or Taq DNA Polymerase
  • Exhibits broad-spectrum RNase inhibitory properties
  • Active over a broad pH range (pH 5–8)
Locations that Product is Available

Asia (only in select countries), Europe, USA

Ordering Information

Name Size Part Package Insert
RNase Inhibitor 2.5KU 62.5µL (2,500U) RNase Inhibitor SIN-201 N/A
RNase Inhibitor 100KU 2.5mL (100KU) RNase Inhibitor (Bulk) SIN-229 N/A
RNase Inhibitor 1,000KU 25mL (1,000KU) RNase Inhibitor (Bulk) SIN-259 N/A

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