This inhibitor exhibits broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, and RNase H. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. This inhibitor can be applied to a reverse transcriptase reaction.
Concentration/Activity**: 40 U/µl
RNase Activity: None detected
Endonuclease Activity: None detected
Exonuclease activity: None detected
** One unit is defined as the amount of RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50 %.
Stabilizes RNA in a cDNA synthesis or a RNA synthesis reaction
Does not inhibit SP6, T7 or T3 RNA Polymerase, AMV or M-MLV Reverse Transcriptase, or Taq DNA Polymerase