Usage
Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “1-step real-time PCR”, including reverse transcription and PCR steps. RNA-direct Realtime PCR Master Mix is applicable for TaqMan assay or hybridization probe assay, in combination with each probe. RNA-direct SYBR Green Realtime PCR Master Mix can be applied to an intercalation assay with SYBR Green I.