RNA-direct Realtime PCR Master Mix

RNA-direct Realtime PCR Master Mix

Origin: Escherichia coli, Recombinant

Usage
Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “1-step real-time PCR”, including reverse transcription and PCR steps. RNA-direct Realtime PCR Master Mix is applicable for TaqMan assay or hybridization probe assay, in combination with each probe. RNA-direct SYBR Green Realtime PCR Master Mix can be applied to an intercalation assay with SYBR Green I.

Key Specifications

• Concentration: 40 reactions / mL
• Functional test*: Correlation coefficient |r | ≥0.98
• Bacterial DNA contamination : Ct ≥ 27
• Ribonuclease Activity : None detected

*Amplification of a G3PDH region from serial dilutions of human G3PDH control RNA and no-template control.

Key Characteristics

• Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination
• Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.)
• Can be used in systems using passive reference (e.g., ABI PRISM 7000, 7700, 7900HT, StepOne Plus, Applied Biosystems, Inc.)
• Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible amplification

Asia (only in select countries), Europe, USA