Realtime PCR Master Mix

Realtime PCR Master Mix

Origin: Escherichia coli, Recombinant

Usage
Realtime PCR Master Mix is applicable in TaqMan assays or hybridization probe assays, in combination with each probe. SYBR Green Realtime PCR Master Mix is applicable for intercalation assay with SYBR Green I. SYBR Green Realtime PCR Master Mix -Plus- is effective for reducing non-specific amplifications or primer dimers in the intercalation assay.

Key Specifications

• Concentration: 40 reactions / mL
• Functional test*: -4.31 ≤ slope ≤ -2.99; correlation factor (r2) ≥ 0.97
• Bacterial DNA contamination**: Ct ≥ 30.

*Amplification test using β-actin gene [295 bp]. Performance on a regression analysis between copy numbers (l logarithm) and Ct using serially diluted genomic DNA solutions.

**Amplification test using16S rDNA [411 bp]. Blank sample analysis.

Key Characteristics

• Bulk format (100mL) also available
• Can be used in glass capillary systems (e.g. LightCycler, Roche Molecular Systems, Inc.)
• Can be used in a passive reference system (e.g., ABI PRISM, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems
• Hot Start technology with anti-TaqDNA polymerase antibodies enables high specificity and reproducible amplification
• Effective for reducing non-specific amplifications or primer dimers

Asia (only in select countries), Europe, USA