KOD exo(-) DNA Polymerase /Anti-KOD Hotstart Antibody
KOD DNA polymerase exhibits an excellent elongation capability (approx. 130 bases/sec) and strong 3’→5’ exonuclease activity (proofreading activity). However, its sensitivity and efficiency are subject to interference from the enzyme or template concentration during PCR, due to its strong proofreading activity. KOD exo(-) is a 3’→5’ exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis.
KOD exo(-) DNA Polymerase /Anti-KOD Hotstart Antibody
EC Number
# 2 . 7 . 7 . 7
Origin: Escherichia coli (R)
Key Specifications
Enzyme Specification
Concentration/Activity*: 2.5 U/μL
Endonuclease Activity: None detected
Nicking Activity: None detected
*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 min at 75°C.
Hotstart Antibody Specification
Source: Mouse Hybridoma
Inhibition Activity**: ≥70%
ssEndonuclease Activity: None detected
dsEndonuclease Activity: None detected
5’ to 3’ Exonuclease Activity: None detected
3’ to 5’ Exonuclease Activity: None detected
Mouse Genomic DNA Contamination: None detected
**Percentage (%) of DNA polymerase activity surpressed when 1.0 μg of antibody is added to 1.25 units of KOD DNA polymerase at 37°C.
Key Characteristics
Excellent processivity & extension ability: Extention time of the thermal cycle can be set <30 seconds / kb.
Great sensitivity & efficiency: PCR sensitivity and efficiency are improved due to elimination of 3’-5’ exonuclease activity.
Hot start PCR: anti-KOD DNA polymerase antibody for the polymerase activity is available.
Can be applied to the real-time PCR: This enzyme can be applied to the real-time PCR assay, except for TaqMan assay due to lacking of the 5’-3’ exonuclease activity. A fast mode cycle can be applied.