KOD exo(-) DNA Polymerase /Anti-KOD Hotstart Antibody

KOD exo(-) DNA Polymerase /Anti-KOD Hotstart Antibody

EC Number

# 2 . 7 . 7 . 7

Origin: Escherichia coli (R)

Key Specifications

Enzyme Specification

• • Concentration/Activity*: 2.5 U/μL
  • Endonuclease Activity: None detected
  • Nicking Activity: None detected

*One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 min at 75°C.

Hotstart Antibody Specification

• • • Source: Mouse Hybridoma
    • Inhibition Activity**: ≥70%
    • ssEndonuclease Activity: None detected
    • dsEndonuclease Activity: None detected
    • 5’ to 3’ Exonuclease Activity: None detected
    • 3’ to 5’ Exonuclease Activity: None detected
    • Mouse Genomic DNA Contamination: None detected

**Percentage (%) of DNA polymerase activity surpressed when 1.0 μg of antibody is added to 1.25 units of KOD DNA polymerase at 37°C.

Key Characteristics

• • • • Excellent processivity & extension ability: Extention time of the thermal cycle can be set <30 seconds / kb.
      • Great sensitivity & efficiency: PCR sensitivity and efficiency are improved due to elimination of 3’-5’ exonuclease activity.
      • Hot start PCR: anti-KOD DNA polymerase antibody for the polymerase activity is available.
      • Can be applied to the real-time PCR: This enzyme can be applied to the real-time PCR assay, except for TaqMan assay due to lacking of the 5’-3’ exonuclease activity. A fast mode cycle can be applied.

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